Figure 3.

The Various functional assessment approaches for Pluripotency. The most stringent tests for the determination of pluripotent cells that includes the various functional assay that essentially rely on the capabilities of test pluripotent cell to self-renew and differentiate into all the three germ cell lineages including ectoderm, mesoderm, and endoderm as mentioned in the text. Some of these assays would also explore the ability of test cells to give rise the whole organism and give rise to a chimeric animal of desired characteristics.