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. 2016 Nov 22;6:36858. doi: 10.1038/srep36858

Figure 2. The characterization of M. ilicifolia friedelin and cycloartenol synthases using heterologous expression in S. cerevisiae.

Figure 2

Total ion chromatograms (TIC) for (a) the standard compounds, including friedelin 1, β-friedelanol 3, α-amyrin acetate 4, β-amyrin acetate 5, epitaraxerol 6, and β-amyrin 2, used in this analysis and for the S. cerevisiae VZL 1303 extracts harbouring (b) empty vector pYES2; (c) vector carrying the coding sequence of friedelin synthase from M. ilicifolia, pYES2-MiFRS; and (d) vector carrying the coding sequence of cycloartenol synthase 1 from M. ilicifolia, pYES2-MiCAS1. The mass spectra with the fragmentation patterns for friedelin 1 and cycloartenol 7 are shown in Supplementary Fig. 1.