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. 2016 Nov 21;47:117. doi: 10.1186/s13567-016-0399-9

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Alignment of the N amino acid sequences of IVI-1173 and of representative PRRSV strains. The 7 amino acid differences between the genotype 1 prototype strain LV and IVI-1173 are shown in blue bold letters. The red star highlights the substitution of the conserved threonine at position 90 of N with an alanine in IVI-1173. Be1 and LA2 (genotype 1) are the closest to IVI-1173 at the nucleotide and amino acid level of N. VR-2332 is the prototype genotype 2 PRRSV. Lena and RVB-581 are highly virulent genotype 1 subtype 3 and genotype 2 strains, respectively. PrimePac (genotype 2) is the only strain for which the lack of N detection by SDOW17 was reported, which is due to substitution of a conserved aspartic acid at position 62 with a tyrosine (red triangle).