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. 2016 Nov 21;47:117. doi: 10.1186/s13567-016-0399-9

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The amino acid at position 90 of PRRSV N is crucial for the epitope recognized by SDOW17. MDM were mock infected (A) or infected with vIVI1173 or the mutant vIVI1173-N(T₉₀) (B, C), with vRVB581 or the mutant vRVB581-N(A₉₀) (D, E) or with CReSA-2982 or LV as controls (F, G). After 18 h, N detection by flow cytometry (left two panels) and by immunofluorescence staining of the monolayers (right two panels) was performed with mAbs SR30 and SDOW17.