Table 3.
Up- and down-regulated P450 genes in cotton bollworm in response to 2-TD treatment
| Family | Classification | Homologous genes | % similarity, organisms | Transcripts number | Log2Ratioa | Expression stage | 2-TD induced |
|---|---|---|---|---|---|---|---|
| P450sb | Mitochondrial | 314A1 | 86%, B. mori | 16487 | 2.55 | All, 3 > 1 > 6 | Up |
| 314A1 | 83%, S. littoralis | 15409 | −2.29 | All, 3 > 1 > 6 | Down | ||
| 333B3 | 69%, S. littoralis | 12317 | −1.12 | All, 1 > 6 > 3 | Down | ||
| 333A3 | 75%, S. littoralis | 25319 | 1.44 | All, 3 > 1 > 6 | Up | ||
| 315A1 | 73%, S. littoralis | 40026 | −1.07 | All, 3 > 1 > 6 | Down | ||
| Clan 2 | 18A1 | 72%, S. littoralis | 41217 | 4.12 | All, 6 = 3 = 1 | Up | |
| 18A1 | 81%, S. littoralis | 35643 | 5.06 | 3 > 1, pupa, female | Up | ||
| 18A1 | 76%, S. littoralis | 16953 | 4.73 | 3 > 1 | Up | ||
| 15C | 71%, B. mori | 14800 | 1.53 | 1, female | Up | ||
| 306A1 | 82%, S. littoralis | 13036 | −3.32 | All, 3 > 1 > 6 | Down | ||
| 307A1 | 99%, H. armigera | 8199 | −4.55 | 3 > 6 > 1, pupa | Down | ||
| 307A1 | 99%, H. armigera | 13311 | −4.92 | Egg, 3 > 6, pupa | Down | ||
| Clan 3 (include CYP6 and CYP9) | 6B2,6B6,6B7 | 96%, H. armigera | 18705 | 3.08 | Egg, female | Up | |
| 6B2, 6B6, 6B7 | 94%, H. armigera | 40306 | 2.23 | All, 3 > 1 > 6 | Up | ||
| 321A2 | 98%, H. zea | 17923 | 7.79 | No expression | Up | ||
| 6B2 | 96%, H. armigera | 2844 | 3.03 | All, 6 > 3 > 1 | Up | ||
| 6B2, 6B6, 6B7 | 97%, H. armigera | 41374 | 4.08 | 3 > 1 > 6, male | Up | ||
| 6B31 | 77%, S. littoralis | 2950 | 1.67 | Male | Up | ||
| 6AB31 | 74%, S. littoralis | 5881 | 2.39 | 1 > 3 > 6, pupa, Adults | Up | ||
| 6B6 | 99%, H. armigera | 39825 | 2.09 | 6 > 3 > 1 | Up | ||
| 6B2, 6B6, 6B7 | 89%, H. armigera | 13679 | 4.38 | 3 | Up | ||
| 6AN4 | 72%, S. littoralis | 12093 | 1.96 | Egg,female,1 > 3 > 6 | Up | ||
| 6AN4 | 78%, S. littoralis | 1833 | 1.07 | 1 < 3 < 6 | Up | ||
| 6AB4 | 74%, B. mori | 42286 | 5.24 | Male | Up | ||
| 6AB14 | 68%, S. littoralis | 15424 | −1.39 | 3 > 6 | Down | ||
| 6AE12 | 72%, H. armigera | 41540 | 2.64 | Female, male | Up | ||
| 6AE12 | 90%, H. armigera | 3564 | 2.35 | 3 > 6 > 1, female | Up | ||
| 6AE14 | 99%, H. armigera | 9094 | −1.55 | 1 > 3 > 6 | Down | ||
| 6AE14 | 78%, H. armigera | 4567 | 1.68 | 1 > 3 > 6, female, male | Up | ||
| 6AE14 | 100%, H. armigera | 15388 | 1.97 | All | Up | ||
| 6AE14 | 92%, H. armigera | 6041 | 1.16 | 3 > 6 > 1 | Up | ||
| 6AE47 | 75%, S. littoralis | 39097 | 2.37 | 6 > 1 > 3, female | Up | ||
| 6AE14 | 72%, H. armigera | 30146 | 3.25 | egg, 3 > 1 > 6, female | Up | ||
| 324A6 | 72%, S. littoralis | 40289 | 2.84 | 3, pupa | Up | ||
| 337B3 | 99%, H. armigera | 2773 | 2.64 | Egg, female | Up | ||
| 337B3v1 | 85%, H. armigera | 12899 | 1.29 | All, 3 > 6 > 1 | Up | ||
| 35D18 | 97%, H. armigera | 17285 | −3.63 | 6 > 3 > 1 | Down | ||
| 35D18 | 91%, H. armigera | 11744 | −4.67 | 6 > 3 > 1 | Down | ||
| 324A1 | 77%, S. littoralis | 36658 | 2.03 | Female, male | Up | ||
| 321A1 | 96%, H. zea | 38041 | 4.82 | Egg, 1 < 3 < 6 | Up | ||
| 321A1 | 95%, H. zea | 6465 | 6.56 | 3 > 6 > 1, male | Up | ||
| 321A2 | 91%, H. zea | 9454 | 2.62 | Egg, female | Up | ||
| 321A2 | 89%, H. zea | 2372 | 1.53 | Egg, pupa, female | Up | ||
| 321B1 | 90%, S. littoralis | 40435 | 1.84 | egg, female | Up | ||
| 9A18 | 88%, H. armigera | 35600 | −3.47 | 6 > 1 > 3, pupa | Down | ||
| 9A18 | 99%, H. armigera | 40298 | −3.49 | All, 6 > 3 > 1 | Down | ||
| 9A18 | 99%, H. armigera | 6517 | −4.46 | 6 > 1 > 3 | Down | ||
| 9A12 | 96%, H. armigera | 16315 | 1.511 | 3 > 1 > 6, female, male | Up | ||
| 9A14 | 94%, H. zea | 13079 | 1.65 | Egg, female | Up | ||
| 337B3v7 | 96%, H. zea | 33786 | 1.50 | Egg, female, 3 > 1 > 6 | Up | ||
| 321A2 | 92%, H. zea | 12163 | 7.21 | 6 > 3 > 1, male | Up | ||
| CYP4 | 4 V2 | 88%, Mamestra brassicae | 17185 | 2.32 | All, 3 > 1 > 6 | Up | |
| 340 K4 | 69%, S. littoralis | 8795 | −1.95 | 3 > 1 > 6 | Down | ||
| 4 M7 | 96%, H. zea | 32914 | 3.18 | 3 > 6 > 1, pupa | Up | ||
| 4 M7 | 97%, H. zea | 34657 | 1.79 | 3 > 6 > 1, pupa | Up | ||
| 4 L12 | 74%, S. littoralis | 41937 | 5.61 | All, 1 > 3 > 6 | Up | ||
| 340AA1 | 66%, S. littoralis | 18087 | 2.03 | Egg, 3 | Up | ||
| 340AA1 | 70%, S. littoralis | 23572 | 1.03 | 3, male | Up | ||
| 4M14V1 | 76%, S. litura | 22567 | 1.50 | Pupa | Up | ||
| 4C1 | 71%, Blaberus discoidalis | 26692 | 1.77 | All | Up | ||
| 4S1 | 96%, H. armigera | 1070 | −1.29 | 6 > 1 > 3,female,pupa | Down | ||
| 367B6 | 73%, S. littoralis | 15813 | 2.03 | 3, pupa, female | Up | ||
| 340AA1 | 70%, S. littoralis | 21273 | 2.03 | 3 | Up | ||
| 4G74 | 83%, S. littoralis | 4194 | −1.91 | 3 > 6 > 1,pupa, female | Down | ||
| 4G15 | D. melanogaster | 2239 | −3.21 | 1 = 3 < 6, female | Down | ||
| 4G74 | 86%, S. littoralis | 14395 | −3.56 | 3 > 6 > 1, pupa, female | Down | ||
| 341B1 | 76%, B. mori | 3370 | −1.92 | 6 > 3 > 1 | Down | ||
| 341B1 | 67%, B. mori | 40986 | −1.19 | Pupa | Down | ||
| 341B3 | 78%, S. littoralis | 7936 | −1.14 | 6 > 3 > 1 | Down | ||
| 341A13 | 83%, S. littoralis | 26038 | −1.55 | 3, female | Up | ||
| 4C1 | 88%, B. mori | 22272 | −2.43 | 6 | Down |
aRatio: RPKM of 2-TD treated samples/RPKM of untreated samples. RPKM: Reads per kilo bases per million reads. RPKM < 0.1 was used as the criterion to judge the unigenes were not expressed during a given developmental stage. Absolute value of Log2Ratio ≥ 1 were used as thresholds for ‘differential expression’. The P450 genes reported to be involved in insect hormone biosynthesis and metabolism are shown in bold. 1: 1st instar larvae; 3: 3rd instar larvae; 6: 6th instar larvae
bThe cytochrome P450 clan schema used here follows the system proposed by Feyereisen et al. (2006)