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Isolation of a truncated form of GK2920. (Left) SDS-PAGE gel of the flowthrough (FT) fraction from the Ni-NTA affinity chromatography column used to purify GK2920. Arrows indicate relevant mass markers (MM; Bio-Rad Precision Plus Blue Standard). The asterisk indicates the predicted size of GK2920WT. (Right) LC/MS of the 17-kDa protein revealed the amino acid sequence highlighted in bold.
