TABLE 3.
Kinetic parametersa of G. kaustophilus and D. radiodurans acetyltransferases
| Enzyme | Substrate | Km(app) (μM) | kcat (s−1) | kcat/Km(app) (M−1 s−1) |
|---|---|---|---|---|
| GK2920M55-Stop | MSX | 121 ± 26 | 9 ± 1 | 7 × 104 |
| MSO | 89 ± 16 | 10 ± 1 | 1 × 105 | |
| PPT | NDAb | NDA | NDA | |
| GK0593 | MSX | 17 ± 4 | 1 ± 0.1 | 6 × 104 |
| MSO | 4 + 2 | 1 ± 0.1 | 3 × 105 | |
| PPT | 5 + 3 | 1 ± 0.1 | 2 × 105 | |
| DR_1182 | MSX | 316 ± 23 | 11 ± 0.3 | 3 × 104 |
| MSO | 198 ± 30 | 11 ± 1 | 6 × 104 | |
| PPT | 721 + 79 | 5 + 0.2 | 7 × 103 |
a
Values represent means ± standard deviations.
b
NDA, no detectable activity.