TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| Bacillus subtilis Y3 | Degrades pendimethalin | 10 |
| Escherichia coli BL21(DE3) | F− ompT hsdS(rB− mB−) gal dcm lacY1 (DE3) | Invitrogen |
| E. coli BL21(DE3)-pnr | E. coli BL21(DE3) harboring the plasmid pET29a-pnr | This study |
| Saccharomyces cerevisiae BY4741 | MATα his3Δ1 leu2Δ met15Δ ura3Δ | This lab |
| Staphylococcus aureus ATCC 25923 | Standard strain of Gram-positive bacteria | This lab |
| Plasmids | ||
| pET29a(+) | Expression vector, Kmra | Novagen |
| pET29a-pnr | pET29a(+) derivative carrying pnr, Kmr | This study |
a
Kmr, kanamycin resistant.