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Purification of PNR from B. subtilis Y3
| Purification step | Total protein (mg) | Total activity (U) | Specific activity (U/mg protein) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Cell extract | 231.62 | 609.53 | 0.38 | 100 | |
| Ammonium sulfate precipitation | 121.82 | 348.41 | 2.86 | 7.53 | 56.56 |
| DEAE-Sepharose chromatography | 37.29 | 241.27 | 6.47 | 17.03 | 39.58 |
| Hydrophobic interaction chromatography | 5.46 | 58.91 | 10.79 | 28.39 | 9.67 |
| Sephadex G-75 gel filtration | 0.12 | 2.22 | 18.5 | 48.68 | 0.36 |
