TABLE 4.
Kinetic constants for PNRa
| Drug | Specific activity (U/mg protein) | Vmax (nmol/min/mg protein) | Km (μM) | Kcat (s−1) | Kcat/Km (μM−1 · s−1) |
|---|---|---|---|---|---|
| NADH | 49.98 | 1.06 × 103 | 35.13 | 375.22 | 10.68 |
| Pendimethalin | 25.11 | 4.39 × 102 | 13.05 | 155.40 | 11.91 |
| Butralin | 23.71 | 3.87 × 102 | 11.54 | 136.99 | 11.61 |
| Oryzalin | 20.22 | 3.76 × 102 | 11.19 | 133.10 | 11.89 |
| Trifluralin | 17.85 | 56.60 | 1.82 | 20.04 | 10.89 |
a
PNR constants for pendimethalin, butralin, oryzalin, and trifluralin calculated with NADH served as electron donors. NADH constants were determined with pendimethalin as the electron acceptor.