FIG 1.

Cellobiose stimulates α-arrestin-dependent internalization of CDT-1 and CDT-2. Cultures of BY4741 (WT) and an otherwise isogenic strain harboring simultaneous deletions of 9 α-arrestin genes (termed 9arrΔ cells) expressing either CDT-1–GFP or CDT-2–GFP were grown, and representative images were obtained in the presence of 2% cellobiose (A) or 2% glucose (B) as described in Materials and Methods. The cells coexpress the intracellular β-glucosidase (GH1-1).