FIG 2.

The α-arrestins Rod1, Rog3, Aly1, and Aly2 cooperate to drive the endocytosis of CDT-1 and CDT-2. (A) Cultures of BY4741 (WT), the 9arrΔ strain, and otherwise isogenic strains with paralogous pairs (or more) of α-arrestin genes deleted were grown and imaged in the presence of 2% cellobiose as described in Materials and Methods. Each strain was cotransformed with the intracellular β-glucosidase (GH1-1) along with either CDT-1–GFP or CDT-2–GFP. (B) The same experiment as that described for panel A was performed, except the cells harbor quadruple deletions of α-arrestin genes.