TABLE 2.
Plasmids used in this study
Plasmid | Description | Reference |
---|---|---|
CDT-1–GFP | PGK1prom CDT-1–GFP–His6 CEN URA3 | This study |
CDT-2–GFP | PGK1prom CDT-2–GFP–His6 CEN URA3 | This study |
GH1-1 | CCW12prom GH1-1 CEN LEU2 | This study |
CDT-2 WT–GFP | CCW12prom CDT-2–GFP–His6 CEN URA3 | This study |
CDT-2 NtKR–GFP | CCW12prom CDT-2(K6R K7R K31R K32R K38R)–GFP–His6 CEN URA3 | This study |
CDT-2 MidKR–GFP | CCW12prom CDT-2(K231R K233R K243R K263R K274R K283R)–GFP–His6 CEN URA3 | This study |
CDT-2 CtKR–GFP | CCW12prom CDT-2 Ct(K484R K493R K512R K522R)–GFP–His6 CEN URA3 | This study |
CDT-2Trunc–GFP | CCW12prom CDT-2Trunc–GFP–His6 CEN URA3 | This study (see Fig. S5) |