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. 2016 Nov 21;82(24):7074–7085. doi: 10.1128/AEM.02148-16

TABLE 3.

Sense-strand sequence of the DNA fragments used to construct the indicated K-to-R mutants of CDT-2

Mutant Description (amino acid changes) DNA
CDT-2 NtKR CDT-2 (K6R K7R K31R K32R K38R) 5′-ATGGGCATCTTCAACAGGCGTCCCGTGGCTCAGGCCGTCGACCTCAATCAGATACAGGAGGAGGCTCCTCAGTTTGAGAGGGTTGACTGGAGAAGGGACCCCGGTCTTCGCAGACTCTACTTCTACGCCTTC
CDT-2 MidKR CDT-2 (K231R K233R K243R K263R K274R K283R) 5′-CCCCGTTTCCTCATCGCCAGAGACAGGCACGACGAGGCCCTCCACATCCTCGCCAGATACCACGCCAACGGCGACCCCAACCACCCCACCGTCCAGTTTGAGTTCCGCGAGATCCGTGAGACCATCCGCCTCGAGATGGAATCGACCCGTAACAGCAGCTACCTCGACTTCTTCAGAAGCCGCGGCAACCGCTAC
CDT-2 CtKR CDT-2 (K484R K493R K512R K522R) 5′-TTCATGTACGTCGAGACCAGAGGCCCCACGCTCGAGGAGCTTGCCAGAGTCATTGATGGCGATGAGGCCGATGTTGCCCACATCGACATTCACCAGGTCGAGAGAGAGGTGGAGATTCACGAGCATGAGGGCAGGTCTGTTGCTGAATTCGAT