TABLE 2.

Primers used to determine the subtypes of NCL1, -2, -3, -7, and -8

Target gene type	Primer direction, sequence (5′–3′)	Annealing temp (°C)	PCR product size (bp)	Description
HG23	Forward, TTTTCAGGGTATGATTCT	45.5	550	NAD-dependent epimerase/dehydratase
	Reverse, CATGTACTTCAGCTATTG
HG55	Forward, TGCTTCATCATCTTCGGT	48.2	846	Hypothetical protein
	Reverse, TGTTGCTTATTAGCCTTG
HG292	Forward, TGACTTTATTGATACCTT	45.3	572	Hypothetical protein
	Reverse, TATTTCTGTTTAGCAGAT
HG293	Forward, TCTTGGTTTCGTTTCTAC	43.5	352	Hypothetical protein
	Reverse, GTTTTTCTTATTTTTGTT
HG294	Forward, AATATAGCTGGGAGTGTC	45.4	300	Hypothetical protein
	Reverse, TGCGGGTATGTTAAAAAG
HG312	Forward, TAGAAGTGCTTATTTTGT	45	384	Integral membrane protein
	Reverse, CTTGAAGTCCATTGCCTG
HG313	Forward, TTGATTAAAGTTGGAGGT	44.3	396	Nucleotidyltransferase family protein
	Reverse, CCCAGATAGAATAGAGCC
HG314	Forward, AAGAGGAGCTTATTGAGG	46	402	Phosphorylcholine transferase
	Reverse, AAATGTCTGCTGTTGTGG
HG315	Forward, AGGTAGACGAACTATCCC	47	506	Choline kinase
	Reverse, CCCCATCTGTAACCAAAA
HG329	Forward, ATGGCTGGCTACTATGCT	48.6	206	Hypothetical protein
	Reverse, AATGTCGTTCCGTTCTCT
HG332	Forward, CCACTACAATAACCCTTC	46.2	968	Hypothetical protein
	Reverse, TAAAACTCCATCTCCTCA
HG354	Forward, TAATGAATCAACGCAAAC	46.1	212	Hypothetical protein
	Reverse, CAAAAGCAGAATAAACCC
HG355	Forward, TCTTGTTGGATTTATTCG	45.3	328	Hypothetical protein
	Reverse, TTAACTTTGGCCTGCTTT
Transposase (NCL1-6)	Forward, GCTTTACCCAATCTATGC	49.2	1,792	Transposase
	Reverse, TGACCTTCTACTGGACCT
Transposase (NCL1-7)	Forward, CAGAAGAGAAGTGGAAGT	47.9	1,446	Transposase
	Reverse, TAGAACAAGTGTGCGATA
Transposase (NCL3-2)	Forward, CATTTAGGATTGACGAAG	48.8	950	Transposase
	Reverse, TTTTACCACCCAACACAG
Transposase (NCL8-3)	Forward, GATAGAGCGAATAGGGTA	48.1	1,020	Transposase
	Reverse, ATTTCAAAAAAAGTGGAC