TABLE 1.

PCR primers and conditions

PCR use	Name	Sequence	Primer annealing temperature (°C)a	Cycle no.	Reference
Bacteria
16S primers for Illumina sequencing	799F-mod3	5′-CMG GAT TAG ATA CCC KGG-3′	52	35	69
	1115R	5′-AGG GTT GCG CTC GTT G-3′			44
HPLC-purified primers to complete Illumina sequencing construct	F	5′-CAA GCA GAA GAC GGC ATA CGA GAT CGG TCT CGG CAT TCC TGC-3′	58	15	44
	R	5′-AAT GAT ACG GCG ACC ACC GAG ATC TAC ACT CTT TCC CTA CAC GAC G-3′			44
Universal bacterial 16S primers	27F	5′-AGA GTT TGA TCM TGG CTC AG-3′	57	35	70
	1492R	5′-CGG TTA CCT TGT TAC GAC TT-3′
Full-length PBE-specific PCR, first half	Burk16SF	5′-TTT TGG ACA ATG GGG GCA AC-3′	50	35	15
	BurkR	5′-TGC CAT ACT CTA GCY YGC-3′			71
Full-length PBE-specific PCR, second half	Burk3-Mod	5′-CGG CGA AAG CCG GAT-3′	50	35	71
	PBE822R-mod	5′-CTW CGT TAC CAA GYC AAT GAA GR-3′			70
Host
16S rRNA	16sa	5′-CGC CTG TTT ATC AAA AAC AT-3′	48	35	72
	16sb	5′-CTC CGG TTT GAA CTC AGA TCA-3′
18S rRNA	18S 1f	5′-TAC CTG GTT GAT CCT GCC AGT AG-3′	48	35	27
	18S 5r	5′-CTT GGC AAA TGC TTT CGC-3′
28S D3-D5 rRNA	D3Fa	5′-TTG AAA CAC GGA CCA AGG AG-3′	48	35	73
	D5Fa	5′-CGC CAG TTC TGC TTA CCA-3′
COI region 1	LCO-1490	5′-GGT CAA CAA ATC ATA AAG ATA TTG G-3′	48	35	74
	HCO-2198	5′-TAA ACT TCA GGG TGA CCA AAA-3′
COI region 2	C1-J-2183	5′-CAA CAT TTA TTT TGA TTT TTT GG-3′	45	35	75
	TL2-N-3014	5′-TCC AAT GCA CTA ATC TGC CAT ATT A-3′

^aPrimer annealing was performed at the indicated temperature for 45 s. All denaturation was performed at 94°C for 30 s, and extension was performed at 72°C for 60 s.