TABLE 1.
Strains used in this study
| Organism | Strain | Description | Source or reference |
|---|---|---|---|
| T. saccharolyticum | LL1025 | Wild-type T. saccharolyticum JW/SL-YS485 | 40 |
| LL1025-0705 | LL1025 ΔxynA::0705 Eryr | This work | |
| LL1025-1705 | LL1025 ΔxynA::1705 Eryr | This work | |
| LL1049 | LL1025 Δpta Δack Δldh adhEG544D | 41 | |
| LL1144 | LL1025 ΔnfnAB::Kanr | 19 | |
| LL1145 | LL1025 Δpta Δack Δldh ΔpyrF ΔnfnAB::Kanr | 19 | |
| LL1305 | LL1025 Δtdk | This work | |
| LL1306 | LL1025 Δtdk Δ1705 | This work | |
| LL1316 | LL1025 Δtdk Δ1705 ΔnfnAB::Kanr | This work | |
| LL1317 | LL1025 Δtdk ΔnfnAB::Kanr | This work | |
| C. thermocellum | LL1004 | Wild-type C. thermocellum strain DSM 1313 | DSMZ |
| LL1087 | LL1004 Δhpt Δrnf | This work | |
| LL1087-1705 | LL1004 Δhpt Δrnf(pDGO126-1705) | This work | |
| E. coli | T7 Express lysY/lq | Used for heterologous protein expression | New England Biolabs |
| DH5α | Used for plasmid screening and propagation | New England Biolabs |