TABLE 2.
Primers used in this study
| Primer | Sequencea | Comment |
|---|---|---|
| LT_01 | CTTTTCCTCCCTCGTCTTC | T.sac_tdk deletion |
| LT_02 | ACTTTTTGTGGTTTTAAACTATTTTCTAAGAGGTGGATTATGGCGGATTTTTAAGGAGGT | |
| LT_03 | TCTTCTTCATTGCTGCACCTCCTTAAAAATCCGCCATAATCCACCTCTTAGAAAATAGTT | |
| LT_04 | GGAATACGCAAAAAGATTG | |
| LT_15 | TACACGTACTTAGTCGCTGAAGCTCTTCTATGAGATACGTTGTTAGAGAAAATAGAG | Amplification of tsac_1705 for plasmid pD861-tsac1705 |
| LT_16 | TAGGTACGAACTCGATTGACGGCTCTTCTACCTCAAAATACTACCTCCCTTGACC | |
| LT_23 | CCACCACAATTCAGCAAA | pD861-tsac1705 sequencing |
| LT_24 | AAAAAACCCCTCAAGACCC | |
| LT_28 | CGATCTCGAGAGATACGTTGTTAGAGAAAATAGAG | Amplification of tsac_1705 for plasmid pDGO126′ins′_p2638-1705 |
| LT_29 | GTCACTCGAGCCGGGTTGAACTACTCTTTAATA | |
| LT_36 | GCAGGCATGCAAGCTTTAATAG | Amplification of vector backbone for plasmid pDGO126′ins′_p2638-1705 |
| LT_37 | AGGTCGACTCTAGAGGATCC | |
| LT_44 | TGATGACGAAAAAGCCGA | pDGO126′ins′_p2638-1705 sequencing |
| LT_45 | ATCCCAATAACCTAACTCTCC | |
| LT_153 | ACGGGAACAATACAAAAGGA | tsac_1705 deletion verification |
| LT_154 | AATTCCTCCCATCCCTATC | |
| LT_155 | TGTGCTGTTGCATGTTGT | tsac_nfnAB deletion |
| LT_156 | GGTGGAGTAATAATTGGTGGT | |
| 0705 F | ATAAATGTGTACATGCCAAAAAAAGTAGAAATATTG | Amplification of tsac_0705 for plasmid pTOPO-0705 |
| 0705 R | CGACCTGCATTAATCGAGAAGTTGCTTTGATTTTGTG | |
| Histag 0705 F | CTGGTTCTCATCATCATCATCATCATGGTATAAATGTGTACATGCCAAAAAAAGTAGAAATATTG | |
| 1705 F | AGATACGTTGTTAGAGAAAATAGAGAAATTAGCAATGG | Amplification of tsac_1705 for plasmid pTOPO-0705 |
| 1705 R | CGACCTGCATCAAAATACTACCTCCCTTGACCAAAATACAGG | |
| Histag 1705 F | CTGGTTCTCATCATCATCATCATCATGGTAGATACGTTGTTAGAGAAAATAGAGAAATTAGCAATGG | |
| pkan 1705 F | GGTCAAGGGAGGTAGTATTTTGATGCAGGTCGATAAACCCAGCG | |
| xynA up F | ATCTTTTCTGGCCTTTAATGGCGC | Amplification of xynA operon and Erm resistance gene for plasmid pTOPO-0705 |
| xynA up R | TGATGATGATGATGATGAGAACCAGACATTCTTACTTCCTCCCTCAGTAAATTTAATTTATTG | |
| pkan 0705 F | CAAAGCAACTTCTCGATTAATGCAGGTCGATAAACCCAGCG | |
| xynA down R | AGTCAAATGCGACAAAAAAACGCC | |
| xynA up R-2 | TCTTACTTCCTCCCTCAGTAAATTTAATTTATTG | |
| pkan F-2 | TGCAGGTCGATAAACCCAGCG | |
| xynA SQ F | GAAATAATTCTAATTCAGTTACCCCG | tsac_0705 or tsac_1705 xynA replacement verification |
| xynA SQ R | GGTGAATTCGAATTTACAGGC |
a
Underlined sequences indicate binding regions.