FIG 1.
Diagram on chromosomal engineering for mevalonate production. Relevant metabolic reactions include glycolysis pathway, mevalonate pathway, and TCA cycle. Red line indicates that ATP inhibits glycolysis, while the green arrow represents that the ATP-limiting condition stimulates glycolysis. The red X represents deletion of the sucA gene, and the black Xs represent homologous recombination of pathway genes. Pi, inorganic phosphate; G6P, glucose 6-phosphate; F6P, fructose 6-phosphate; DHAP, dihydroxyacetone phosphate; G3P, glyceraldehyde 3-phosphate; 3PG, 3-phosphoglyceric acid; PEP, phosphoenolpyruvic acid; PYR, pyruvate.