TABLE 1.
Primers used in this study
| Primer name | Primer sequence (5′ to 3′) | Location |
|---|---|---|
| mvaEF | CGGTAAGGATCCAGGAGAAATTAACTATGAAATTTTACGAG | Forward primer to amplify the mvaE gene plus BamHI site |
| mvaERadhE1 | CGTTTATGTTGCCAGACAGCGCTACTGATTAATCCCGATTTTCATCTTTTGATTG | Reverse primer to amplify the mvaE gene plus 28 nt of DNA homologous to the downstream of the adhE gene |
| mvaERadhE2 | CTTTTCTAGAATCGGCATTGCCCAGAAGGGGCCGTTTATGTTGCCAGACAGCGCTACTGA | 50 nt of DNA homologous to the downstream of the adhE gene plus XbaI site |
| CmF | TCGTCTTCACCTCGAGGTAGTCAATAAACCGGTAAACCAGC | Forward primer to amplify the cm gene |
| CmR | ACGTTGTTATCTCTTGTCAACACCGCCAGAGATAACCTGTGACGGAAGATCACTTCGCAG | Reverse primer to amplify the cm gene plus 35 nt of the M1-93 promoter sequence |
| Pm1 | TTATCTCTGGCGGTGTTGACAAGAGATAACAACGTTGATATAATTGAGCC | Primer to amplify the M1-93 promoter |
| Pm2 | GACAAGAGATAACAACGTTGATATAATTGAGCCCGTATTGTTAGCATGTACGTTTAAAC | Primer to amplify the M1-93 promoter |
| Pm3 | AGTTAATTTCTCCTGTTTAAACGTACATGCTAACAATACGGGCTCAATTATATCAACGTT | Reverse primer to amplify the M1-93 promoter |
| atoF | GTTTAAACAGGAGAAATTAACTATGAAAAATTGTGTCATCGTCAGTGC | 22 nt of the M1-93 sequence plus the forward sequence to amplify the atoB gene |
| atoR | AATCCCCATATGATAACCATGGGTGGCGCACATC | Reverse primer to amplify the first 480 bp of the atoB gene, including NdeI site |
| CmLig1 | TCTAGTTGTGCAAAACATGCTAATGTAGCCACCAAGTAGTCAATAAACCGGTAAACCAGC | 35 nt of DNA homologous to the upstream of the adhE gene plus forward sequence to amplify the cm gene |
| CmLig2 | TCTTCACCTCGAGAAATTGCTATCATTCGTTATTGTTATCTAGTTGTGCAAAACATGC | 45 nt of DNA homologous to the upstream of the adhE gene plus XhoI site |
| atpF1 | CAATCCTCGGCCAGGCCATCGCGTTTGTCCTGTTTGTAGGCTGGAGCTGCTTCG | 34 nt of DNA homologous to the upstream of the atpF gene plus forward sequence to amplify the kan cassette |
| atpR1 | GTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCATTCCGGGGATCCGTCGACC | Reverse sequence to amplify the kan cassette plus 35 nt of DNA homologous to the downstream of the atpH gene |
| atpF2 | GTGAATCTTAACGCAACAATCCTCGGCCAGGCCATCGCGTTTGTCCTGTTTGTAG | Forward primer to add 50 nt of DNA homologous to the upstream of the atpF gene |
| atpR2 | TTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCATTCC | Reverse primer to add 50 nt of DNA homologous to the downstream of the atpH gene |