TABLE 2.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or descriptiona | Reference or source |
|---|---|---|
| Strains | ||
| BW25113 | Δ(araD-araB)567ΔlacZ4787(::rrnB-3) ΔlacZ4787(::rrnB-3) Δ(rhaD-rhaB)568 hsdR514 | CGSC |
| JW0855-1 | ΔpoxB::kan | CGSC |
| JW2294-1 | Δpta::kan | CGSC |
| CMEV-1 | BW25113 ΔadhE::cm-M1-93-atoB-mvaS-mvaE | This study |
| CMEV-2 | BW25113 ΔldhA::kan-M1-93-atoB-mvaS-mvaE | This study |
| CMEV-3 | BW25113 ΔadhE::cm-M1-93-atoB-mvaS-mvaE ΔatpFH::kan | This study |
| CMEV-4 | BW25113 ΔadhE::cm-M1-93-atoB-mvaS-mvaE ΔatpFH::FRT ΔpoxB::kan | This study |
| CMEV-5 | BW25113 ΔadhE::cm-M1-93-atoB-mvaS-mvaE ΔatpFH::FRT Δpta::kan | This study |
| CMEV-6 | BW25113 ΔadhE::cm-M1-93-atoB-mvaS-mvaE ΔldhA::FRT-M1-93-atoB-mvaS-mvaE ΔatpFH::kan | This study |
| CMEV-7 | BW25113 ΔadhE::cm-M1-93-atoB-mvaS-mvaE ΔldhA::FRT-M1-93-atoB-mvaS-mvaE ΔsucA ΔatpFH::kan | This study |
| Plasmids | ||
| pMEV-7 | pZE-PLlacO1-atoB-mvaS-mvaE | 5 |
| pKD13 | kan cassette | CGSC |
| pKD46 | λ Red recombinase expression | CGSC |
| pCP20 | FLP recombinase expression | CGSC |
| pMEV-10 | pZE-PLlacO1-atoB-mvaS-mvaE-50-nt homolog | This study |
| pMEV-11 | pZE-60 nt-homolog-cm-M1-93-atoB-mvaS-mvaE-50-nt homolog | This study |
a
FRT, Flp recombinase target; nt, nucleotide.