FIG 4.

sEPS^neg is not protective in a T cell transfer colitis model. Following receipt of CD4⁺ CD25⁻ CD45RB^hi T cells, C.B-17 SCID mice were orally administered B. longum 35624 (n = 8), sEPS^comp (n = 8), or sEPS^neg (n = 8). (A) Weight loss and disease activity were monitored over time. Statistical significance was determined using two-way ANOVA (*, P < 0.05). (B) Following euthanasia, the colon/body weight ratio was determined. A representative picture of colons from B. longum 35624- and sEPS^neg-treated animals is provided. (C) The percentages of IL-17⁺, IFN-γ⁺, and IL-10⁺ lymphocytes from mesenteric lymph nodes are illustrated (n = 8 mice per group). Data are presented as box-and-whisker plots with the median values and maximum/minimum values illustrated. Statistical significance was determined using the Kruskal-Wallis test and Dunn's multiple-comparison test (*, P < 0.05 for sEPS^neg strain versus the other strains). Error bars show standard deviations.