Table 1.

Simulated yields of products as a function of experimental parameters

Conditions	pH	7.4	7.4	7.4	7.4	7.4	7.4
	[GSH]/mM	10	10	10	0	10	10
	[Hasc-]/mM	1	0.1	0	0.1	0.1	0
	[O2]/μM	30	30	30	30	200	200
		Calculated yield/initial protein thiyl radical (in %)
Product yield	GSSGa	0.2	12	30	0	12	32
	(Thio-)aldehydeb	3.6	34	62	18	32	52
	D-Cys in Protein	7.5	16	133	39	20	33
	Protein peroxyl radical	0.5	2	7.9	23	8	13
	Ascorbyl radicalc	96	78	0	79	47	0
	O2•− d	3.7	20	44	18	44	84
	GSSG(total)e	51	64	64	0	70	94
	Protein damage (sum)	12	52	203	80	60	98

^avia oxidation of GSSG^•−

^bvia oxidation of alpha-mercaptoalkyl radicals of Cys by O₂

^cvia reaction with GS^• and RS^•, reacts with O₂ to form O₂^•−

^dvia a) and b), dismutates to form H₂O₂

^evia a), and oxdation by protein hydroperoxide, H₂O₂, and reduction of protein peroxyl radicals either by ascorbate or GSH