Figure 3.

Glucagon receptor gene transcripts in patient and normal liver. (A) Multiplex PCR analysis of cDNA from the patient and from normal human liver using a primer set, which leads to amplification of a 343 bp fragment including sequences encoded within exons 7–10. (B) Sequence analysis of cloned glucagon receptor cDNA from the patient and from normal human liver verifying the deletion of exon 9. Sequence analysis of the intron 8/exon 9 splice junction. (C) Autoradiogram comparing the sequence of the non-coding strand across the splice junction of exon 9 in cloned glucagon receptor genomic DNA from the patient with the corresponding sequence obtained from cloned wild-type human glucagon receptor genomic DNA. (D) Schematic diagram of the human glucagon receptor gene structure showing the position of the invariant AG dinucleotide in intron 8 and the G → A substitution.