FIG 2.

Nucleoside analog incorporation catalyzed by human POLRMT. Representative denaturing PAGE gels are shown indicating reaction products from POLRMT-catalyzed nucleotide incorporation with the indicated nucleoside analog triphosphate and RNA primer/DNA template. Reactions were allowed to proceed at room temperature for 5 min. RNA primer was extended to n + 1 in the presence of each nucleoside triphosphate with the exception of UTP (multiple misincorporations observed). Percentage incorporations of the respective nucleoside triphosphates are shown {based on [(band intensity of upper incorporated RNA product)/(total band intensities of upper incorporated RNA product and lower unincorporated RNA primer)] × 100}. The assay was repeated a minimum of three times to calculate the mean percentages of incorporation and standard deviations shown in Table 3.