FIG 2.

Comparison of in vitro antiplasmodial activity, serum stability, and cytotoxicity of compound 1e (A) and the pantetheinase-susceptible isobutyl pantothenamide (B) under various conditions. (A) Dose-response relationship of compound 1e in the presence (black circles) and absence (white triangles) of pantetheinase. The effect of increasing the concentration of pantothenate (from 1 μM to 100 μM) in the presence of pantetheinase on the antiplasmodial activity of compound 1e was also tested (gray circles). The white circles show the lack of an effect of compound 1e on the proliferation of HFF cells. (B) Isobutyl pantothenamide activity against the parasite was tested in the presence (black circles) and absence (white triangles) of pantetheinase. This assay served as a positive control and was carried out in paired experiments with compound 1e. The data are averaged from 2 to 9 independent experiments, each carried out in triplicate. Error bars represent the range divided by 2 (if n = 2) or SEM (if n ≥ 3).