Relative viability of Caco-2 and CHO cells exposed to different concentrations of ClyR. Cells were cultured in DMEM supplemented with 10 to 15% fetal bovine serum, 1% penicillin, and 1% streptomycin for 24 h. After the cells were exposed to a series of concentrations of ClyR (0, 125, 250, 500, 1,000, 2,000 μg/ml) for another 24 h, the relative viability of the cells after each treatment was determined by the CCK-8 assay.