Figure 6. Salubrinal-pretreatment exacerbates the cytotoxicity of lithocholic acid (LCA) in PC-3 and DU-145 prostate cancer cells (24 h exposure).
Statistically significant differences in cell viability between salubrinal-treated and vehicle control-treated cells were observed by two-way ANOVA and Bonferroni post-hoc test (* p < 0.05; *** p < 0.001). Experiments were performed in triplicate using different cell passages; per experiment each concentration was tested in triplicate.