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Thermal stress and phase transition of anti CD40 antibody as a function of heating rate (SE corresponds to fit error).
| Instrumentation | Buffer | pH (Initial) | Heating Rate (°C/hr) | Tm ± S.E. (°C) |
|---|---|---|---|---|
| Fluorescence | PBS | 7.0 | 60 | 64.5 ± 0.6 |
| CD | PBS | 7.0 | 30 | 68.3 ± 0.4 |
| CD | PBS | 7.0 | 60 | 69.1 ± 0.3 |
| CD | PBS | 7.0 | 120 | 70.5 ± 0.8 |
| CD | Acetate | 4.0 | 30 | 68.1 ± 1.8 |
| CD | Acetate | 4.0 | 60 | 69.1 ± 1.4 |
| CD | Acetate | 4.0 | 120 | 70.4 ± 2.0 |
