Fig. 1.

Typical PARR electropherograms showing polyclonal and clonal products. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Size markers are shown in red. A: Polyclonal products from two separate IgH reactions from the same sample. Fragments are multiplexed for GeneScan analysis with the shorter FWR3 fragments (blue; primer set 1) on the left and the longer FWR1 fragments (green; primer set 4) on the right. There is a Gaussian distribution of products with a peak every three bases. B: Clonal products from a B-cell lymphoma using the same primer sets as in A. A single tall sharp peak is seen. Occasionally a smaller secondary peak is seen at the base to the left; this feature is due to overloading of the capillary with product and disappears if the product is diluted. C: Polyclonal TCRγ products (blue; primer set 7) exhibiting a Gaussian distribution without a peak every 3 bases. A slightly irregular jagged appearance can sometimes occur. D: Clonal TCRγ product (primer set 7) with a similar appearance to the IgH peaks in B.