Table 2.
Antibodies used for flow cytometric analysis of canine samples .
| Antibody | Cellular Target | Clone |
|---|---|---|
| Isotype-matched triple negative FITC/PE/Alexa647 | Negative control | |
| CD5-FITC | Pan T-cell | YKIX322.3 |
| CD21-PE | Mature B-cell | CA2.1D6 |
| CD45-Alexa647 | Pan leukocyte | YKIX716.13 |
| CD3-FITC | Pan T-cell | CA17.2A12 |
| CD4-PE | Helper T-cell | YKIX302.9 |
| CD8-Alexa647 | Cytotoxic T-cell | YCATE55.9 |
| CD34-FITC | Stem/precursor cells | 2E9 |
| CD79a-Alexa647a | Pan B-cell | HM57 |
| MHC II-FITC | Activated lymphocytes | YKIX334.2 |
| MAC387-FITCa | Macrophages and monocytes | MAC387 |
| CD14-Alexa647 | Monocytes | TUK4 |
FC antibodies were sourced from AbD Serotec, with the exception of CD34-FITC (Santa Cruz Biotechnology Inc, Heidelberg, Germany).
a
Antibody targeting an internal epitope (requires permeabilisation).