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Reaction of reduced MtCDH‐heme with CBP21‐Cu(II). A: Oxidation of 5 µM MtCDH by CBP21 (15, 25, and 50 µM) was followed in a stopped‐flow spectrometer at 563 nm. Observed electron transfer rates are plotted in (B). Partially reduced MtCDH was obtained by reduction with cellobiose, in 50 mM sodium phosphate buffer, pH 6.0. Error bars show the standard deviation of three replicates. Concentrations are those after mixing.
