Figure 6.

Degradation of β‐chitin by CBP21 and oxidation of lactose by MtCDH. A: Time course analysis of degradation of 10 mg/mL β‐chitin (equivalent to 24.6 mM chitobiose) by 1.0 µM CBP21 using either 1.5 µM MtCDH and 3.0 mM lactose (squares) or 1.0 mM ascorbate (triangles) as reducing agents. B: Oxidation of lactose by 1.5 µM MtCDH in the presence (squares) or absence (triangles) of 1.0 µM CBP21. C: Degradation of β‐chitin by 1.0 µM CBP21 in the presence of 1.5 µM MtCDH, 3.0 mM lactose and 0, 0.02, or 0.2 µM ChiC. All reactions contained 10 mg/mL β‐chitin. The standard deviations for all experiments are shown by error bars (n = 3). All reactions (panels A, B, and C) were buffered in 25 mM Bis‐Tris, pH 6.0.