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Specific Activity (k iso) and k cat/K m Value of Refolded Synthetic Calstabin Versus Recombinant Calstabin
| k iso [s−1] (×10−2)a | k cat/K m [mM−1 s−1]b | |
|---|---|---|
| Recombinant calstabin (protein 1) | 23.3 ± 0.06 | 442 ± 29 |
| Synthetic calstabin (protein 1) | 23.8 ± 0.1 | 438 ± 11 |
| Chymotrypsin only | 1.03 |
Recorded at 0.52 µM for calstabin concentration.
Calculated for enzyme concentrations between 0.4 and 1 µM.
