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k cat/K m Value of Synthetic Native and Refolded Mutant Calstabin Versus Recombinant Calstabin
| Enzyme | k cat/K m (mM−1 s−1) |
|---|---|
| Recombinant calstabin (protein 1) | 438 ± 17 |
| Synthetic calstabin (protein 1) | 438 ± 17 |
| R13 (N‐ω‐methyl‐l‐arginine) calstabin (protein 5) | 470 ± 41 |
| K18 (Nε‐(2,4‐dinitrophenyl)‐l‐lysine) calstabin (protein 4) | 508 ± 18 |
| TAMRA‐calstabin (protein 6) | 494 ± 37 |
Calculate + d for enzyme concentrations between 0.4 and 1 µM.
