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. 2016 Sep 23;25(12):2142–2151. doi: 10.1002/pro.3038

Figure 1.

Figure 1

Expression of UspF protein by an IPTG‐inducible E. coli BL21 (DE3) pLyS strain and purification of soluble fractions containing UspF protein. A – T0 total protein extract before induction; T2, total protein extract after induction (3 h); T3, total protein extract after induction (16–18 h). The arrow indicates position of the UspF protein (18.4 kDa). B – Purification of soluble fractions or UspF protein by affinity chromatography using a nickel‐containing resin. 1–Flow through; 2–30 mM, 3–50 mM, 4–100 mM; 5–200 mM of imidazole.