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. 2016 Sep 23;25(12):2142–2151. doi: 10.1002/pro.3038

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© 2016 The Authors Protein Science published by Wiley Periodicals, Inc. on behalf of The Protein Society

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Bacterial lysates from strains Ec292/84, 9100/83, BA320 and BA4013 submitted or not to stress conditions. LB; LB pH 4.8; 3 M NaCl; 0.045% of H₂O_2; LB at 42°C (10 µg) were separated by SDS‐PAGE (12,5%) and transferred to a nitrocellulose membrane. Nitrocellulose membranes were incubated with anti‐UspF rabbit polyclonal serum followed by goat anti‐rabbit IgG peroxidase‐conjugate. Immunodetection signals were visualized by addition of DAB/H₂O₂.