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Structure and purity of NECA and adenosine nucleotides. (A) Chemical structures of NECA, adenosine, and ADP. (B) Reverse‐phase chromatographic analysis of ligands used in this study. NECA has a unique retention time and no measurable contamination of ATP, ADP, AMP, or adenosine. The inset shows trace contamination of AMP and ATP in ADP stock solutions.
