Fig. 6.

Ex-vivo human skin imaging-comparison of home-built MPM-based imaging platform with a commercial system using the same objective. (a) Dermo-epidermal junction (DEJ) imaged with the home-built microscope by SHG (blue) and TPEF (green). TPEF signal originates from keratin in the epidermal keratinocytes and from elastin fibers (arrows) in the superficial papillary dermis, while SHG highlights the collagen fibers. (b) A similar location of the DEJ in the skin sample acquired with a commercial Olympus microscope over an area of 370 x 370 µm² by using the same objective as in the home-built microscope. (c) MPM image of the DEJ acquired with the home-built microscope over an area of 370 x 370 µm² for comparison with the image in (b) acquired with the Olympus microscope. The image in (c) corresponds to the inset in (a). (d) MPM image of the DEJ corresponding to the inset in (c) showing keratinocytes (full line arrows), elastin fibers (arrow heads) and collagen fibers (dashed line arrows). Images were acquired at 50µm depth in the sample. Scale bar is 100 µm.