Table 1. Basic imaging properties of the scan head.
| Property | Specification | Comment |
|---|---|---|
| Frame rate | 800 x 800 pixels: 10 fps-single frame to 0.8 fps-12 frames averaged for SNR>20 1600 x 1600 pixels: 5 fps-single frame to 0.4 fps- 12 frames averaged for SNR>20 |
High frame rate for rapid browsing of tissue |
| Frame dimension | 800x800 to1600x1600 | Image dimension controllable by software |
| FOV | 0.8 x 0.8 mm2 | Wide FOV covers larger tissue areas |
| Lateral translation | 1.0 cm | Enables mesoscale exploration of tissue |
| Resolution | 0.5 µm lateral; 3.3 µm axial | High resolution |
| Imaging depth | 0.100-0.250 mm | Sufficient to capture epidermis and reach the dermis |
| Detection channels | TPEF; SHG | NLO signals detected with high sensitivity PMTs |
| Bright field | LED illuminator plus CCD | Bright field reflection detected in epi-mode |