Fig 2. Reduced H3K27me3 binding is detected by ChIP-qPCR.

(A) ChIP was performed using chromatin from KARPAS-422 cells treated with the EZH2 inhibitor CPI-360. qPCR using the positive control primer MYT1 showed reduced H3K27me3 occupancy in the presence of the inhibitor. (B) ChIP was performed using chromatin from PC9 cells treated with the EZH2 inhibitor GSK126. qPCR using the positive control primer MYT1 showed reduced H3K27me3 occupancy in cells treated with the inhibitor. (C) Libraries were generated from KARPAS-422 cells using 15 cycles of PCR amplification. Library DNA was diluted and qPCR was performed using positive control primers for MYT1 and CCND2. (D) Libraries were generated from PC9 cells as described in (C) and library DNA was used for qPCR using positive control primers for MYT1 and CCND2. All experiments are represented as the mean of two independent experiments with qPCRs performed in triplicate ±SD. The ACTB promoter served as a negative control for all experiments.