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. 2016 Nov 22;11(11):e0166763. doi: 10.1371/journal.pone.0166763

Fig 2. Calmodulin, CaMKII, CKIα and PlkI inhibitors reduced HCV NS5A S235 phosphorylation in the HEK293T cells.

Fig 2

(A-D) Representative and (E-H) summary of the immunoblotting for NS5A and NS5A phosphorylation at S235 (pS235) in the NS3-5A transfected HEK293T cells. One day after the transfection, the cells were exposed to the inhibitors for 2 days before the immunoblotting analysis. Values are Mean ± SEM (n = 3). Asterisk indicates significance i.e. p<0.05, t-test against the values in the vehicle controls (0 μM). Protein intensities were measured with the Li-Cor Odyssey scanner and software and adjusted for the loadings with the β-actin intensity. Relative abundance was normalized against the values under the vehicle control (0 μM). (I-L) Cell viability assessed with the MTT assay. The cells were exposed to the inhibitors for 2 days before the viability assay. Values are Mean ± SEM (n = 3).