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. 2016 Nov 22;11(11):e0166763. doi: 10.1371/journal.pone.0166763

Fig 6. CKIα knockdown reduced NS5A S235 phosphorylation and HCV RNA levels in the infected Huh7.5.1 cells.

Fig 6

(A) Viability of the Huh7.5.1 cells with or without CKIα knockdown assessed with the MTT assay. (B) Representative and summary (C) of the immunoblotting for CKIα, NS5A and NS5A phosphorylation at S235 (pS235) in the HCV-infected Huh7.5.1 cells with or without CKIα knockdown. One day after the cells were infected with HCV (J6/JFH1), the cells were subjected to shRNA-based CKIα knockdown for 6 days prior to the immunoblotting analysis. Values are Mean ± SEM (n = 3). Asterisk indicates significance i.e. p<0.05, t-test against the values in the non-targeted control cells (shCon). Protein abundance was quantified with the Li‐Cor scanner, adjusted for the loadings (i.e. against β-actin) and normalized with the values in the control cells. (D) CKIα mRNA and HCV RNA levels measured with quantitative RT-PCR in the HCV-infected Huh7.5.1 cells with or without CKIα knockdown. The experiments were done as those in b except that RNA was collected for the analysis. The GAPDH mRNA levels were analyzed as loading controls. Relative RNA abundance was normalized with the values of the control cells.