Table 2. RT-PCR primers used for CaMKII isoform analysis.
Primer | Type | Sequence 5’ – 3’ |
---|---|---|
CaMKIIα F | exon-exon junction | TGGAATCCTCAGAGAGCACC |
CaMKIIα R | exon-exon junction | CGCACATCTTCGTGTAGGACT |
CaMKIIβ F | exon-exon junction | AGTCAAGCCCCAGACGAATA |
CaMKIIβ R | exon-exon junction | GCACTGTCAGAAGACTCCTT |
CaMKIIγ F | exon-exon junction | GTGGAGTGTTTGCGCAAGTT |
CaMKIIγ R | exon-exon junction | TGACACCGCCATCCGACT |
CaMKIIδ F | exon-exon junction | GGAATTTCTCAGCAGCCAAG |
CaMKIIδ R | exon-exon junction | GCTTTCGTGCTTTCACATCT |
CaMKIIα F | exon primer | ATCGCCTACATCCGCATC |
CaMKIIα R | exon primer | CCAGATCTGTGGAAGTGGAC |
CaMKIIβ F | exon primer | GTTGTTCCTGCTTTCCCTTC |
CaMKIIβ R | exon primer | TTTCAATACTCCAGGGGGTG |
The exon-exon primers span two neighboring exons whereas the exon primers are within the same exon of a gene. F, forward primer; R, reverse primer.