Table 3. Plasmids used for shRNA-mediated gene knockdown.
| shRNA clone (ID) | Sequence | Target |
|---|---|---|
| shCon (TRCN0000072246) | CAAATCACAGAATCGTCGTAT | no target in the Huh7.5.1 cells |
| shCKIα(TRCN0000342507) | GCAGAATTTGCGATGTACTTA | coding region |
| shCaMKIIγ (1) (TRCN0000000476) | CGTTGCTGTACTGTCTTGTTT | 3’ UTR |
| shCaMKIIγ (2) (TRCN0000000478) | AGAACAGCAAGCCTATCCATA | coding region |
| shCaMKIIδ (1) (TRCN0000195619) | CCATGGATCTGTCAACGTTCT | coding region |
| shCaMKIIδ (2) (TRCN0000000474) | CAGATGGAGTAAAGGAGTCAA | coding region |
| shCaMKIIδ (3) (TRCN0000000475) | TGTGGTGTCATTCTCTATATT | coding region |
CaMKII, calmodulin-dependent kinase II; CKIα, casein kinase I α; shCon, non-targeting control shRNA plasmid.