Table 1.
Epigenetic effector domains used for targeted epigenetic editing
| Gene regulation | Epigenetic effector | Enzymatic activity | Chromatin modification | Genes targeted |
|---|---|---|---|---|
| Repression | G9a | Methyltransferase | H3K9me2 | VEGF-A, Her2INeu, Fosb, E-Cadherin, Neruog, Grm2 |
| Suv39h1 | Methyltransferase | H3K9me3 | VEGF-A, Her2INeu, Neruog, Grm2 | |
| DNMT3 (A, A/L) | Methyltransferase | DNA methylation | VEGF-A, SOX2, Maspin, EpCAM, CDKN2A, ARF, Cdkn1a,IL6ST, BACH2 | |
| LSD1 | Demethylase | H3K4me2 | Gene enhancers | |
| SIRT6, SIRT3 | Deacetylase | H3K9ac | Neruog, Grm2 | |
| KYP | Methylase | H3K9me1 | Neruog, Grm2 | |
| TgSET8 | Methylase | H3K20me | Neruog, Grm2 | |
| NUE | Methylase | H3K27me3 | Neruog, Grm2 | |
| HDAC8 | Deacetylase | H4K8ac | Neruog, Grm2 | |
| RPD3 | Deacetylase | H4K8ac | Neruoq, Grm2 | |
| Sir2a | Deacetylase | H4Kac | Neruoq, Grm2 | |
| Sin3a | Deacetylase | H3K9ac | Neruog, Grm2 | |
| Activation | TET1 | Deoxygenase | DNA demethylation | ICAM-1, RHOXF2, BRCA1, RANKL, MAGEB2, MMP2 |
| TET2 | Deoxygenase | DNA demethylation | ICAM-1, EpCAM | |
| TET3 | Deoxygenase | DNA demethylation | ICAM-1 | |
| TDG | Glycosylase | DNA demethylation | Nos2 | |
| p300 | Acetylase | H3K27ac | IL1RN, MYOD1, OCT4, HBE, HBG,ICAM-1 | |
| PRDM9 | Methyltransferase | H3K4me3 | EpCAM,ICAM-1, RASSF1a, PLOD2 | |
| Dot1L | Methyltransferase | H3K79me | EpCAM, PLOD2 |