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. 2016 Nov 22;11(11):e0167135. doi: 10.1371/journal.pone.0167135

Fig 5. CUR increases the expression of SmCaspase 3/7 and the activity of Caspase 3 in adult S. mansoni adult worms, but it does not increase the activity of Caspase 8.

Fig 5

Couples of adult worms were incubated with CUR at 25 and 50 μM for 24 h. After incubation, female and male S. mansoni worms were separated and analyzed. In the negative control groups, couples of adult worms were incubated with RPMI 1640 medium with 0.1% DMSO. Relative levels of expression of SmCASP3, SmCASP7, and SmCASP8 transcripts in adult (A) female and (B) male worms incubated with CUR. Expression was calculated according to the comparative Ct method (2-ΔΔCT method), and data were normalized relative to an endogenous standard gene (SmGPDH). The activities of Caspase 3 and 8 were measured as described in material and methods. The activity of Caspase 3 referred to adult (C) female and (D) male worms. The activity of caspase 8 referred to adult (E) female and (F) male worms. Values are expressed as the mean ± S.E.M of three independent experiments. An asterisk indicates statistically significant differences as compared to the negative control group (RPMI 1640 medium with 0.1% DMSO) (**p < 0.01, ***p < 0.001).