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. 2016 Nov 21;215(4):543–558. doi: 10.1083/jcb.201601090

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© 2016 Scharaw et al.

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Figure 4. — EGF stimulation up-regulates COPII paralogues in HeLa and A431 cells. HeLa cells and A431 cells were stimulated for 12 or 24 h with 200 ng/ml EGF, PDGF, or IGF. The effect on COP paralogue expression levels was investigated by Q-RT-PCR and targeted proteomics. Q-RT-PCR results were normalized to nontreated cells and GAPDH mRNA levels. Targeted proteomics results were normalized to nontreated cells and prelamin A/C protein levels. (A) Log2 mRNA fold changes of COP paralogues and EGFR in HeLa cells treated for 12 h with EGF (n = 4). (B) Log2 mRNA fold changes of COPII paralogues in A431 cells treated for 24 h with EGF, PDGF, or IGF (n = 6). (C) Basal log2 mRNA fold change expression of COPII paralogues in nontreated A431 cells compared with nontreated HeLa cells (n = 3). Data in A–C are means ± SEM (t test; *, P < 0.05; **, P < 0.01; ***, P < 0.001). (D) Log2 protein abundances of COPII paralogues in HeLa cells treated for 24 h with EGF (n = 3). Data are medians ± median absolute deviation.