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. 2016 Nov 21;215(4):483–498. doi: 10.1083/jcb.201604015

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© 2016 Martin et al.

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Figure 6. — pix-1and pak-1 control the formation of polarized protrusions by dorsal anterior cells. z-projections of confocal images and spreading rates of protrusions produced by dorsal cells are indicated over time (bottom) for three representative wt, pix-1(gk416), pak-1(ok448), and let-502(RNAi) (A) and pix-1(gk416); let-502(RNAi) (B) embryos expressing AJM-1::GFP (green; top) and VAB-10(ABD)::mCherry (red; top). Bars: 10 µm; (enlarged images) 5 µm. The dashed lines correspond to mean spreading rates for wt (black), pix-1(gk416) (blue), and let-502(RNAi) (green). (C) Violin plot representing the distribution and mean spreading rates for N = 4 embryos and n = 96 measurements. t test p-values significantly different from wt (*) and pix-1(gk416) (#) are indicated in parentheses. (D) Anteroposterior distribution of protrusions produced by dorsal cells. The black line corresponds to the distribution observed in wt embryos. p-values of Fisher exact test are indicated.