Figure 2.

Auditory and vestibular function is reduced in mice with mutations in Pls1 and Fscn2. (A) Auditory brainstem response (ABR) thresholds. dB pe SPL, decibel peak-equivalent sound-pressure level. For both ABRs and DPOAEs, the number of animals (2 mo old) tested were 8 C57BL/6, 6 Pls1^+/+, 11 Pls1^−/−, 7 Fscn2^R109H/R109H, and 10 Pls1^−/− Fscn2^R109H/R109H. (B) Distortion-product otoacoustic emission (DPOAE) amplitudes. Frequency plotted is the geometric mean of f₁ and f₂. Dashed gray line represents the mean noise floor measured across all studies. (C) VsEP responses for stimuli of the indicated amplitudes (in decibels, referenced to 1 g/ms). Cross-linker symbols at the bottom of each panel indicate which proteins are functional (gray is nonfunctional or absent). p1, p2, and n1 of the VsEP response are indicated. (D) VsEP thresholds are mildly elevated in Fscn2^R109H/R109H mice and moderately elevated in Pls1^−/− and Pls1^−/− Fscn2^R109H/R109H mice (*, P < 0.05; ***, P < 0.001). In A and B, error bars indicate SD. In D, boxes indicate 25–75% range, white lines indicate mean, and solid colored bars indicate data range (except outliers, which are indicated by points and connected with gray dashed lines). Numbers of animals tested for each genotype are shown; all animals were 2 mo old.